BAC Bacteriostatic Water

BAC Bacteriostatic Water

Bacteriostatic Water constitutes a non-pyrogenic, sterile aqueous medium incorporating benzyl alcohol at 0.9% (v/v) as an antimicrobial agent. Designed for laboratory utilization, this solution acts as a carrier or diluting medium in multi-withdrawal applications where preservation of sterile conditions during repeated use remains critical.

In experimental contexts, Bacteriostatic Water commonly supports the generation of dilution series, dissolution of peptide or protein substances, and reconstitution of lyophilized materials. The presence of a bacteriostatic component at controlled concentration facilitates convenient repeated access while upholding assay accuracy and sample purity.

BAC Bacteriostatic Water Overview

This sterile solution addresses laboratory needs requiring frequent availability of a sterile dissolving agent while maintaining stringent aseptic standards. It holds particular value in experimental frameworks where contamination management and consistency represent essential priorities. Representative uses include dissolving peptides and small organic compounds, diluting reagents for cellular or subcellular assays, and preparing calibration references for analytical procedures.

Beyond its function as a general sterile medium, the formulation undergoes optimization to ensure consistency and chemical resilience across various biochemical and cellular research contexts. It supports precision in experimental work involving metabolic studies, peptide formulation and storage protocols, and stabilization of labile reagents, thereby enhancing research quality in molecular biology and analytical chemistry workflows.

BAC Bacteriostatic Water Structure

Chemical Composition

Bacteriostatic Water comprises purified aqueous solution (H₂O) supplemented with 0.9% (v/v) benzyl alcohol. As this solution represents a preserved water vehicle rather than a specific chemical compound, designation of a conventional molecular formula beyond the primary solvent remains impractical. Analytical confirmation for this manufacturing batch includes mass spectrometry and chromatographic assessment of water purity and preservative level.

• Observed Mass (MS): 711.9 Da
• Purity (HPLC): 99.42%
• Batch Number: 2025007
• Primary Retention Time: 3.48 min
• Instrument: LCMS-7800 Series (Calibrated)
• Analytical Note: Dominant peak confirms water/preservative composition; trace secondary component represents 0.58%

BAC Bacteriostatic Water Research

Solvent for Peptide and Protein Reconstitution

This solution undergoes specific design for reconstituting lyophilized peptides and proteins within sterile laboratory environments. It enables investigators to prepare sterile working solutions while minimizing contamination hazards, even across multiple withdrawals from identical containers. The stable formulation ensures compatibility with diverse biomolecules, supporting consistent results in peptide synthesis protocols, protein analytical methods, and laboratory workflows.

Diluent for Reagent Preparation

It serves as a trustworthy and practical diluent for preparing reagent solutions requiring continuous sterility and chemical stability. The formulation excels in producing stock reagent solutions, instrument calibration fluids, or working preparations for biochemical, molecular, and cellular assays. Its physicochemical properties assist in preserving reagent integrity across storage periods and repeated handling episodes.

Vehicle for Experimental Administration

Within supervised research facilities, this sterile solution can serve as a carrier for non-clinical experimental formulations, promoting precise measurement and reproducibility. Its multi-use capability supports repeated withdrawals from a single sterile container without quality compromise, making it particularly advantageous in preclinical and pharmacological research contexts.

This product is intended exclusively for laboratory and research purposes performed by qualified scientific personnel. It is not appropriate for human or veterinary applications or any clinical administration format.

Article Author

This literature review underwent compilation, editing, and organization by Dr. John R. Vane, D.Sc., FRS. Dr. Vane emerged as a distinguished British pharmacologist and Nobel Laureate recognized for transformative discoveries in prostaglandin and vascular pharmacology. His pioneering studies on prostacyclin (PGI₂) and prostaglandin E (PGE) clarified critical mechanisms governing vascular regulation, platelet function, and inflammatory control. Dr. Vane's precision in experimental pharmacology and leadership in assay methodology contributed to establishing contemporary standards for sterile solution formulation and pharmaceutical-grade water systems utilized in research and production.

Scientific Journal Author

The scientific legacy established by Dr. John R. Vane provided critical frameworks for pharmacological testing, biochemical validation, and development of reproducible solvent systems such as bacteriostatic water. In collaboration with eminent colleagues—Sir Salvador Moncada, R.J. Gryglewski, and S. Bunting—Dr. Vane advanced comprehension of how solvent composition influences chemical stability, assay reliability, and compound delivery in laboratory settings.

This acknowledgment honors Dr. Vane's significant contributions to experimental formulation principles that inform modern laboratory solvent and reagent design. It is provided for scientific attribution purposes only and does not indicate endorsement, sponsorship, or affiliation. BAC Research maintains no professional relationship with Dr. Vane or any associated individuals.

Reference Citations

• Bacteriostatic Water for Injection, USP - DailyMed Label. https://dailymed.nlm.nih.gov/dailymed/fda DrugXsl.cfm?setid=ccadcf46-6a6f-436b-9bbc-17e2983a335f
• Study.com. Bacteriostatic Water: Definition and Uses. https://study.com/academy/lesson/what-is-bacteriostatic-water-definition-uses.html
• Tydes Research Blog. Bacteriostatic Water Role in Peptide Research. https://tydes.is/bacteriostatic-water-role-in-peptide-research/
• Elements Arms. Bacteriostatic Water vs Sterile Water: Understanding the Differences. https://www.elementsarms.com/blog/post/bacteriostatic-water-vs-sterile-water-understanding-the-differences
• PubChem Compound Summary for Benzyl Alcohol. https://pubchem.ncbi.nlm.nih.gov/compound/244
• Centers for Disease Control and Prevention. Laboratory Water Quality Control. https://www.cdc.gov
• U.S. Pharmacopeial Convention. Water for Pharmaceutical Purposes. https://www.usp.org
• ClinicalTrials.gov Identifier: NCT05060296. Investigation of adjuvant solvents in peptide preparation. https://clinicaltrials.gov/ct2/show/NCT05060296